TY - JOUR
T1 - A benchmarking protocol for breath analysis: the peppermint experiment
AU - Henderson, Ben
AU - Ruszkiewicz, Dorota M.
AU - Wilkinson, Max
AU - Beauchamp, Jonathan D.
AU - Cristescu, Simona M.
AU - Fowler, Stephen J.
AU - Salman, Dahlia
AU - Francesco, FD
AU - Koppen, Gudrun
AU - Langejürgen, Jens
AU - Holz, Olaf
AU - Hadjithekli, Andria
AU - Moreno, Sergi
AU - Pedrotti, Michele
AU - Sinues, Pablo
AU - Slingers, Gitte
AU - Wilde, Michael
AU - Lomonaco, Tommaso
AU - Zanella, Delphine
AU - Zenobi, Renato
AU - Focant, Jean François
AU - Grassin-Delyle, Stanislas
AU - Franchina, Flavio Antonio
AU - Malásková, Michaela
AU - Stefanuto, Pierre Hugues
AU - Pugliese, Giovanni
AU - Mayhew, Christopher
AU - Thomas, C. L.Paul
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Abstract
Sampling of volatile organic compounds (VOCs) has shown promise for detection of a range of diseases but results have proved hard to replicate due to a lack of standardization. In this work we introduce the ‘Peppermint Initiative’. The initiative seeks to disseminate a standardized experiment that allows comparison of breath sampling and data analysis methods. Further, it seeks to share a set of benchmark values for the measurement of VOCs in breath. Pilot data are presented to illustrate the standardized approach to the interpretation of results obtained from the Peppermint experiment. This pilot study was conducted to determine the washout profile of peppermint compounds in breath, identify appropriate sampling time points, and formalise the data analysis.
Five and ten participants were recruited to undertake a standardized intervention by ingesting a peppermint oil capsule that engenders a predictable and controlled change in the VOC profile in exhaled breath. After collecting a pre-ingestion breath sample, five further samples are taken at 2, 4, 6, 8, and 10 h after ingestion. Samples were analysed using ion mobility spectrometry coupled to multi-capillary column and thermal desorption gas chromatography mass spectrometry. A regression analysis of the washout data was used to determine sampling times for the final peppermint protocol, and the time for the compound measurement to return to baseline levels was selected as a benchmark value. A measure of the quality of the data generated from a given technique is proposed by comparing data fidelity.
This study protocol has been used for all subsequent measurements by the Peppermint Consortium (16 partners from seven countries). So far 1200 breath samples from 200 participants using a range of sampling and analytical techniques have been collected. The data from the consortium will be disseminated in subsequent technical notes focussing on results from individual platforms.
AB - Abstract
Sampling of volatile organic compounds (VOCs) has shown promise for detection of a range of diseases but results have proved hard to replicate due to a lack of standardization. In this work we introduce the ‘Peppermint Initiative’. The initiative seeks to disseminate a standardized experiment that allows comparison of breath sampling and data analysis methods. Further, it seeks to share a set of benchmark values for the measurement of VOCs in breath. Pilot data are presented to illustrate the standardized approach to the interpretation of results obtained from the Peppermint experiment. This pilot study was conducted to determine the washout profile of peppermint compounds in breath, identify appropriate sampling time points, and formalise the data analysis.
Five and ten participants were recruited to undertake a standardized intervention by ingesting a peppermint oil capsule that engenders a predictable and controlled change in the VOC profile in exhaled breath. After collecting a pre-ingestion breath sample, five further samples are taken at 2, 4, 6, 8, and 10 h after ingestion. Samples were analysed using ion mobility spectrometry coupled to multi-capillary column and thermal desorption gas chromatography mass spectrometry. A regression analysis of the washout data was used to determine sampling times for the final peppermint protocol, and the time for the compound measurement to return to baseline levels was selected as a benchmark value. A measure of the quality of the data generated from a given technique is proposed by comparing data fidelity.
This study protocol has been used for all subsequent measurements by the Peppermint Consortium (16 partners from seven countries). So far 1200 breath samples from 200 participants using a range of sampling and analytical techniques have been collected. The data from the consortium will be disseminated in subsequent technical notes focussing on results from individual platforms.
U2 - 10.1088/1752-7163/aba130
DO - 10.1088/1752-7163/aba130
M3 - Article
SN - 1752-7155
VL - 14
SP - 46008
EP - 46008
JO - Journal of Breath Research
JF - Journal of Breath Research
IS - 4
ER -