Electron microscopical analysis of drug effects on brain tumour cell cell physiology

Glioblastoma multiforme (GBM) is the deadliest brain tumour, effective treatment options for which remain elusive. The current treatment procedure of maximal resection followed by chemotherapy proved to be grossly insufficient to prevent the disease progression and death. Despite best efforts, the maximum survival post-diagnosis is a mere 1.5 year. Therefore, there is a huge unmet clinical need to find effective therapeutic procedures to prevent the pathogenesis and relapse of GBM. Small molecule inhibitors of JAK signalling pathway have shown promising outcome in preventing GBM cell proliferation in a previous study (Smedley and Patra, 2023) . We aim to extend that, and in this project, we would like to analyse the morphological and structural changes in GBM cells induced by JAK inhibitors by using the Scanning Electron Microscopy.

(i) Impact of various JAK inhibitors in controlling GBM.
(ii) Modulation of JAK inhibitor efficiency in combination with nanoparticles to control GBM.
(iii) SEM analysis of the morphological and structural changes induced by the JAK inhibitors in GBM cells.